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Issue 1, 2004
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Arsenic–glutathione complexes—their stability in solution and during separation by different HPLC modes

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Abstract

Complexes of arsenic compounds and glutathione are believed to play an essential part in the metabolism and transport of inorganic arsenic and its methylated species. Up to now, the evidence of their presence is mostly indirect. We studied the stability and chromatographic behaviour of glutathione complexes with trivalent arsenic: i.e. AsIII(GS)3, MAIII(GS)2 and DMAIII(GS) under different conditions. Standard ion chromatography using PRP X-100 and carbonate or formic acid buffer disintegrated the complexes, while all three complexes are stable and separable by reversed phase chromatography (0.1% formic acid/acetonitrile gradient). AsIII(GS)3 and MAIII(GS)2 were more stable than DMAIII(GS), which even under optimal conditions tended to degrade on the column at 25 °C. Chromatography at 6 °C can retain the integrity of the samples. These results shed more light on the interpretation of a vast number of previously published arsenic speciation studies, which have used chromatographic separation techniques with the assumption that the integrity of the arsenic species is guaranteed.

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Publication details

The article was received on 11 Jul 2003, accepted on 24 Sep 2003 and first published on 21 Oct 2003


Article type: Paper
DOI: 10.1039/B307945G
Citation: J. Anal. At. Spectrom., 2004,19, 183-190
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    Arsenic–glutathione complexes—their stability in solution and during separation by different HPLC modes

    A. Raab, A. A. Meharg, M. Jaspars, D. R. Genney and J. Feldmann, J. Anal. At. Spectrom., 2004, 19, 183
    DOI: 10.1039/B307945G

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