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Volume 126, 2004
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Imaging capabilities of synchrotron infrared microspectroscopy

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Abstract

It has become increasingly clear that synchrotron infrared micro-spectroscopy is an extremely valuable analysis tool when determining the chemical composition of biological and biomedical samples, at the diffraction-limited spatial resolution. Highly resolved infrared micro-spectroscopy, together with the high signal-to-noise level of the recorded spectra, is essential in generating chemical and statistical (multivariate) images. This is illustrated in the case of individual cell and hair section studies. Unprecedented chemical images of lipid distribution and secondary structure relative concentration have been achieved using the synchrotron source. A comparison with a Focal plane Array imaging system, on the same hair section, shows that, despite the fast imaging processing and improved quality achieved with the focal plane array detectors, spectral quality is markedly superior in the case of the synchrotron source. It is clear that the two approaches could be very complementary if combined on the same sample area, in a synchrotron facility.

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Publication details

The article was received on 06 May 2003, accepted on 15 Jul 2003 and first published on 15 Oct 2003


Article type: Paper
DOI: 10.1039/B305065C
Citation: Faraday Discuss., 2004,126, 289-302
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    Imaging capabilities of synchrotron infrared microspectroscopy

    P. Dumas, N. Jamin, J. L. Teillaud, L. M. Miller and B. Beccard, Faraday Discuss., 2004, 126, 289
    DOI: 10.1039/B305065C

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