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Issue 7, 2004
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On-line atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

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Abstract

A new technique is presented for the coupling of atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) mass spectrometry with liquid delivery systems. Mass measurements of polymers and peptides are demonstrated using a co-dissolved matrix, e.g. α-cyano-4-hydroxycinnamic acid (HCCA). Improvements in terms of sensitivity are achieved by optimizing the shape und control of the exit capillary and by using a laser (355 nm) at a 1 kHz repetition rate. Two calibration experiments promise a good applicability of the presented coupling method for quantitative measurements. The limit of detection achieved so far is 500 nM for peptides in methanol solution containing 25 mM HCCA.

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Publication details

The article was received on 19 Mar 2004, accepted on 30 Apr 2004 and first published on 07 Jun 2004


Article type: Paper
DOI: 10.1039/B404178J
Citation: Analyst, 2004,129, 574-578
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    On-line atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

    J. M. Daniel, S. Ehala, S. D. Friess and R. Zenobi, Analyst, 2004, 129, 574
    DOI: 10.1039/B404178J

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