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Issue 10, 2004
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A decade of vibrational micro-spectroscopy of human cells and tissue (1994–2004)

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Instrumentation used in infrared microspectroscopy (IR-MSP) permits the acquisition of spectra from samples as small as 100 pg (10−10 g), and as small as 1 pg for Raman microspectroscopy (RA-MSP). This, in turn, allows the acquisition of spectral data from objects as small as fractions of human cells, and of small regions of microtome tissue sections. Since vibrational spectroscopy is exquisitely sensitive to the biochemical composition of the sample, and variations therein, it is possible to monitor metabolic processes in tissue and cells, and to construct spectral maps based on thousands of IR spectra collected from pixels of tissue. These images, in turn, reveal information on tissue structure, distribution of cellular components, metabolic activity and state of health of cells and tissue.

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Publication details

The article was received on 11 Jun 2004, accepted on 13 Aug 2004 and first published on 27 Aug 2004

Article type: Critical Review
DOI: 10.1039/B408952A
Citation: Analyst, 2004,129, 880-885
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    A decade of vibrational micro-spectroscopy of human cells and tissue (1994–2004)

    M. Diem, M. Romeo, S. Boydston-White, M. Miljković and C. Matthäus, Analyst, 2004, 129, 880
    DOI: 10.1039/B408952A

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