Jump to main content
Jump to site search

Issue 4, 2003
Previous Article Next Article

Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation

Author affiliations

Abstract

A highly effective, reagentless, mechanical cell lysis device integrated in microfluidic channels is reported. Sample preparation, specifically cell lysis, is a critical element in ‘lab-on-chip’ applications. However, traditional methods of cell lysis require purification steps or complicated fabrication steps that a simple mechanical method of lysis may avoid. A simple and effective mechanical cell lysis system is designed, microfabricated, and characterized to quantify the efficiency of cell lysis and biomolecule accessibility. The device functionality is based on a microfluidic filter region with nanostructured barbs created using a modified deep reactive ion etching process. Mechanical lysis is characterized by using a membrane impermeable dye. Three main mechanisms of micro-mechanical lysis are described. Quantitative measurements of accessible protein as compared to a chemically lysed sample are acquired with optical absorption measurements at 280 and 414 nm. At a flow rate of 300 µL min−1 within the filter region total protein and hemoglobin accessibilities of 4.8% and 7.5% are observed respectively as compared to 1.9% and 3.2% for a filter without nanostructured barbs.

Back to tab navigation

Publication details

The article was received on 08 May 2003, accepted on 30 Jul 2003 and first published on 28 Aug 2003


Article type: Paper
DOI: 10.1039/B305162E
Citation: Lab Chip, 2003,3, 287-291
  •   Request permissions

    Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation

    D. D. Carlo, K. Jeong and L. P. Lee, Lab Chip, 2003, 3, 287
    DOI: 10.1039/B305162E

Search articles by author

Spotlight

Advertisements