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Issue 1, 2003
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Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter

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Abstract

The integration of complete analyses systems “on chip” is one of the great potentials of microfabricated devices. In this study we present a new pressure-driven microfabricated fluorescent-activated cell sorter chip with advanced functional integration. Using this sorter, fluorescent latex beads are sorted from chicken red blood cells, achieving substantial enrichments at a sample throughput of 12000 cells s−1. As a part of the sorter chip, we have developed a monolithically integrated single step coaxial flow compound for hydrodynamic focusing of samples in flow cytometry and cell sorting. The structure is simple, and can easily be microfabricated and integrated with other microfluidic components. We have designed an integrated chamber on the chip for holding and culturing of the sorted cells. By integrating this chamber, the risk of losing cells during cell handling processes is eliminated. Furthermore, we have also developed integrated optics for cell detection. Our new design contributes to the ongoing efforts for building a fully integrated micro cell sorting and analysing system.

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Publication details

The article was received on 23 Sep 2002, accepted on 03 Jan 2003 and first published on 23 Jan 2003


Article type: Paper
DOI: 10.1039/B209333B
Citation: Lab Chip, 2003,3, 22-27
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    Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter

    A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter and P. Telleman, Lab Chip, 2003, 3, 22
    DOI: 10.1039/B209333B

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