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Issue 4, 2003
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Nonlinear decrease of background fluorescence in polymer thin-films – a survey of materials and how they can complicate fluorescence detection in μTAS

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Abstract

Polymers and plastics are receiving increased attention as materials for microfluidics and μTAS applications. Given the ubiquity of fluorescence detection techniques in micro-analytical systems, the fluorescence properties of polymers and plastics should not be overlooked. We survey some commonly available polymer thin-films for their fluorescence behaviour under standardized conditions to determine which materials are most suitable for high-sensitivity fluorescence detection lab chips. The initial fluorescence intensities of some of the materials surveyed were significantly higher than glass and fused silica controls, and decreased over the three hour period with complex kinetics. We then discuss how this has confounded fluorescence detection in our analytical context, and possible mechanisms for the decrease.

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Publication details

The article was received on 08 Jul 2003, accepted on 28 Aug 2003 and first published on 18 Sep 2003


Article type: Paper
DOI: 10.1039/B307772C
Citation: Lab Chip, 2003,3, 248-252
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    Nonlinear decrease of background fluorescence in polymer thin-films – a survey of materials and how they can complicate fluorescence detection in μTAS

    K. R. Hawkins and P. Yager, Lab Chip, 2003, 3, 248
    DOI: 10.1039/B307772C

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