Jump to main content
Jump to site search

Issue 2, 2003
Previous Article Next Article

Estrogenic chemicals and estrogenic activity in leachate from municipal waste landfill determined by yeast two-hybrid assay

Author affiliations

Abstract

Estrogenic activity and estrogenic chemicals in landfill leachate were investigated by yeast two-hybrid assay and chemical analysis. Leachate sample extracted by liquid–liquid extraction with dichloromethane at pH 7.0 showed a higher dose–response curve than sample extracted at pH 3.0, or than sample extracted by solid phase extraction at either pH 7.0 or 3.0. The fraction extracted at pH 3.0 specifically inhibited not only growth of yeast but also estrogenic activity in this assay, suggesting that it contained anti-estrogenic chemicals. The greatest contributor to estrogenic activity among the chemicals identified in leachate extract was bisphenol A, with an estimated contribution ratio of 84%. The contribution ratios of 4-nonyl phenol (4-np) and 4-tert-octyl phenol (4-t-op) were estimated at 1.0% and 0.1%, respectively, while natural estrogens such as 17β-estradiol or estrone were below detection limit, so that their contribution ratio was estimated at no more than 10%. The estrogenic activity of leachate was decreased by aeration treatment alone after 7 days, and was no longer detected after 22 days. Concentrations of bisphenol A, 4-np and 4-t-op likewise decreased with aeration.

Back to tab navigation

Publication details

The article was received on 07 Nov 2002, accepted on 07 Jan 2003 and first published on 23 Jan 2003


Article type: Paper
DOI: 10.1039/B210962J
Citation: J. Environ. Monit., 2003,5, 269-274
  •   Request permissions

    Estrogenic chemicals and estrogenic activity in leachate from municipal waste landfill determined by yeast two-hybrid assay

    Y. Kawagoshi, Y. Fujita, I. Kishi and I. Fukunaga, J. Environ. Monit., 2003, 5, 269
    DOI: 10.1039/B210962J

Search articles by author

Spotlight

Advertisements