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Issue 11, 2003
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Correlation of acid–base chemistry of phytochelatin PC2 with its coordination properties towards the toxic metal ion Cd(II)

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Abstract

The acid–base properties and cadmium binding abilities of (γ-Glu-Cys)2-Gly (PC2), a short phytochelatin, were studied in solution, using potentiometry, 1H NMR and UV-vis spectroscopy. Macroscopic and microscopic constants established by potentiometry and NMR allowed the complete dissociation processes of the hexaprotic PC2 molecule to be explained. The stoichiometry of the complexes formed by phytochelatin with cadmium depends very strongly on reactant ratios. For different amounts of ligand with respect to the metal ion the CdH2L and CdHL species exist in almost the same molar fractions. Above pH 6 complexes with one (CdL) or two ligand molecules bound (CdH3L2, CdH2L2, CdHL2 and CdL2, respectively) were found depending on the Cd(II) ∶ peptide ratio. 1H NMR and UV-vis spectroscopy show coordination of four sulfur atoms from two molecules of PC2 to one cadmium(II) ion (RCd–S: 2.52 Å). Amino groups, glutamic acid and glycine deprotonated carboxyls also participate in cadmium coordination, in contrast to thiolate groups, in monomeric complexes in acidic solutions. Quantitative comparison of metal ion binding strength by PC2 with low molecular weight peptide thiols (LMWT), glutathione and its fragments show that over a wide range of pH phytochelatin binds to cadmium(II) ions several times more strongly than LMWT.

Graphical abstract: Correlation of acid–base chemistry of phytochelatin PC2 with its coordination properties towards the toxic metal ion Cd(ii)

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Publication details

The article was received on 04 Feb 2003, accepted on 01 Apr 2003 and first published on 24 Apr 2003


Article type: Paper
DOI: 10.1039/B301357J
Citation: Dalton Trans., 2003,0, 2253-2259
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    Correlation of acid–base chemistry of phytochelatin PC2 with its coordination properties towards the toxic metal ion Cd(II)

    V. Dorčák and A. Krężel, Dalton Trans., 2003, 0, 2253
    DOI: 10.1039/B301357J

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