Jump to main content
Jump to site search

Issue 23, 2003
Previous Article Next Article

Supramolecular functional interfacial architectures for biosensor applications

Author affiliations

Abstract

This short review describes some of our efforts in generating bio-functional supramolecular interfacial architectures for their use as affinity coatings in biosensor development based on a recently introduced novel optical recording principle combining the resonant excitation of surface plasmon modes and fluorescence detection schemes. Examples are given for multilayer assemblies designed for surface hybridization reactions between a grafted oligonucleotide catcher probe and target strands from solution. By describing the binding behavior of fluorophore-labeled rabbit–anti-mouse IgG to surface-attached mouse IgG, the limit of detection of the surface plasmon fluorescence spectroscopy will be shown to be in the lower femtomolar concentration range. These DNA-and protein binding studies will be complemented by examples for membrane-based biosensor platforms. We will document that tethered lipid bilayer membranes can be assembled with specific capacities of Cm = 0.5μF cm−2 and specific resistivities in excess of Rm > 20 MΩ cm2. The incorporation of the synthetic ionophore valinomycin can mediate the K+-ion translocation across these tethered membranes, thus reducing the resistivity selectively and reversibly by more than four orders of magnitude.

Back to tab navigation

Publication details

The article was received on 26 Aug 2003, accepted on 23 Oct 2003 and first published on 03 Nov 2003


Article type: Paper
DOI: 10.1039/B310317J
Citation: Phys. Chem. Chem. Phys., 2003,5, 5169-5175
  •   Request permissions

    Supramolecular functional interfacial architectures for biosensor applications

    W. Knoll, F. Yu, T. Neumann, S. Schiller and R. Naumann, Phys. Chem. Chem. Phys., 2003, 5, 5169
    DOI: 10.1039/B310317J

Search articles by author

Spotlight

Advertisements