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Issue 3, 2002
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Generation of concentration gradient by controlled flow distribution and diffusive mixing in a microfluidic chip

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Abstract

We have developed a simple method to generate a concentration gradient in a microfluidic device. This method is based on the combination of controlled fluid distribution at each intersection of a microfluidic network by liquid pressure and subsequent diffusion between laminas in the downstream microchannel. A fluid dynamic model taking into account the diffusion coefficient was established to simulate the on-chip flow distribution and diffusion. Concentration gradients along a distance of a few hundred micrometers were generated in a series of microchannels. The gradients could be varied by carefully regulating the liquid pressure applied to the sample injection vials. The observed concentration gradients of fluorescent dyes generated on the microfluidic channel are consistent with the theoretically predicted results. The microfluidic design described in this study may provide a new tool for applications based on concentration gradients, including many biological and chemical analyses such as cellular reaction monitoring and drug screening.

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Publication details

The article was received on 29 Jan 2002, accepted on 22 Apr 2002 and first published on 14 May 2002


Article type: Paper
DOI: 10.1039/B201021F
Citation: Lab Chip, 2002,2, 158-163
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    Generation of concentration gradient by controlled flow distribution and diffusive mixing in a microfluidic chip

    M. Yang, J. Yang, C. Li and J. Zhao, Lab Chip, 2002, 2, 158
    DOI: 10.1039/B201021F

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