Issue 5, 2002
From the journal:

Chemical Communications

Butane and propane oxidation by engineered cytochrome P450cam

Stephen G. Bell,a   Julie-Anne Stevenson,a   Helen D. Boyd,a   Sophie Campbell,a   Austin D. Riddle,a   Erica L. Ortona  and  Luet-Lok Wong*a  

* Corresponding authors

a Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK

Abstract

The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme–substrate fit: the F87W/Y96F/T101L/V247L mutant oxidises butane with a turnover rate of 750 min−1 and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min−1 with 4% yield.

Graphical abstract: Butane and propane oxidation by engineered cytochrome P450cam

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Article information

DOI
https://doi.org/10.1039/B110957J
Article type
Communication
Submitted
29 Nov 2001
Accepted
18 Jan 2002
First published
07 Feb 2002

Chem. Commun., 2002, 490-491

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Butane and propane oxidation by engineered cytochrome P450cam

S. G. Bell, J. Stevenson, H. D. Boyd, S. Campbell, A. D. Riddle, E. L. Orton and L. Wong, Chem. Commun., 2002, 490 DOI: 10.1039/B110957J

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