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Issue 6, 2002
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An electrochemical adaptation of Ellman’s test

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The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2–120 µM, a limit of detection of 1.17 µM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.

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Publication details

The article was received on 19 Mar 2002, accepted on 25 Apr 2002 and first published on 15 May 2002

Article type: Paper
DOI: 10.1039/B202780C
Citation: Analyst, 2002,127, 797-802
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    An electrochemical adaptation of Ellman’s test

    O. Nekrassova, P. C. White, S. Threlfell, G. Hignett, A. J. Wain, N. S. Lawrence, J. Davis and R. G. Compton, Analyst, 2002, 127, 797
    DOI: 10.1039/B202780C

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