The determination of protein phosphorylation on electrophoresis gel blots by laser ablation inductively coupled plasma-mass spectrometry

Abstract

Laser ablation interfaced with inductively coupled plasma-mass spectrometry is described as a new method to determine the presence of phosphorylated proteins on electrophoresis gel blots. The method was applied to the phosphoprotein β-casein with good detection levels being observed at 16 pmole. Attempts at using the technique to detect β-casein on electrophoresis gels are also described.