Jump to main content
Jump to site search

Issue 2, 2002
Previous Article Next Article

Mixed enzyme (laccase/tyrosinase)-based remote electrochemical biosensor for monitoring phenolic compounds

Author affiliations

Abstract

An electrochemical biosensor for remote continuous monitoring of phenolic compounds in environmental analysis is described. The probe relies on rapid and sensitive amperometric detection at a submersible biosensor assembly, connected to a 50 ft long shielded cable. The enzymes laccase and tyrosinase were used as individual sensors and also as a bienzymatic sensor; these enzymes were immobilized chemically on the carbon fiber transducer. The analysis was based on the amperometric detection of the enzymatic products at a potential of −0.10 V vs. Ag/AgCl. Operational conditions were optimized to meet the requirements of remote operations. Tests with untreated river water spiked with phenolic compounds gave results similar to those obtained with synthetic buffer solutions. The remote laccase biosensor allowed the convenient quantification of guaiacol and chloroguaiacol at levels down to 22 and 9 nmol L−1, respectively. The co-immobilization of laccase and tyrosinase allowed the efficient detection of a larger group of phenolic compounds.

Back to tab navigation

Publication details

The article was received on 02 Nov 2001, accepted on 03 Dec 2001 and first published on 11 Jan 2002


Article type: Paper
DOI: 10.1039/B110011D
Citation: Analyst, 2002,127, 258-261
  •   Request permissions

    Mixed enzyme (laccase/tyrosinase)-based remote electrochemical biosensor for monitoring phenolic compounds

    R. S. Freire, S. Thongngamdee, N. Durán, J. Wang and L. T. Kubota, Analyst, 2002, 127, 258
    DOI: 10.1039/B110011D

Search articles by author

Spotlight

Advertisements