Jump to main content
Jump to site search

Issue 8, 2002
Previous Article Next Article

Biocatalytic precipitation induced by an affinity reaction on dendrimer-activated surfaces for the electrochemical signaling from immunosensors

Author affiliations

Abstract

We have developed a strategy of signal generation for immunosensors that transduces biospecific affinity recognition reactions into electrochemical signals. The cyclic voltammetric method, tracking the precipitation of insoluble products onto the sensing surface and the subsequent decrement in the electrode area, was chosen for signal registration. Precipitation of insolubilities was induced by the catalytic reaction of enzymes, which were labeled to the biospecifically attached protein or antibody molecules. As a model system for affinity recognition, we have investigated the functionalization of biotin groups to the sensing monolayer and their biospecific interactions with anti-biotin antibody molecules. The immunosensing interface was developed onto the dendrimer-activated self-assembled monolayers (SAMs), as the base template for the functionalization of the antigen moiety and signal generation. The advantages of using dendrimer-activated SAMs in comparison to the plain modified thiolate SAMs for the sensing surface were shown in terms of sensing performances, and the analytical characteristics of the resulting immunosensor were examined. Additionally, the sensing system was applied for biotin/(strept)avidin couples, extending the applicability of the developed strategy.

Back to tab navigation

Publication details

The article was received on 03 Apr 2002, accepted on 05 Jun 2002 and first published on 11 Jul 2002


Article type: Paper
DOI: 10.1039/B203299F
Citation: Analyst, 2002,127, 1082-1087
  •   Request permissions

    Biocatalytic precipitation induced by an affinity reaction on dendrimer-activated surfaces for the electrochemical signaling from immunosensors

    H. C. Yoon, H. Yang and Y. T. Kim, Analyst, 2002, 127, 1082
    DOI: 10.1039/B203299F

Search articles by author

Spotlight

Advertisements