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Issue 24, 2001
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Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution

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Abstract

The combination of error prone PCR at high mutation rate and DNA shuffling can be used to invert the direction of enantioselectivity of a lipase-catalyzed hydrolytic kinetic resolution involving a chiral ester.

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Publication details

The article was received on 30 Oct 2001, accepted on 15 Nov 2001 and first published on 06 Dec 2001


Article type: Communication
DOI: 10.1039/B109913M
Citation: Chem. Commun., 2001,0, 2664-2665
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    Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution

    D. Zha, S. Wilensek, M. Hermes, K. Jaeger and M. T. Reetz, Chem. Commun., 2001, 0, 2664
    DOI: 10.1039/B109913M

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