Issue 8, 2001
From the journal:

Analyst

Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD)

Mifumi Shimomura,a   Yoko Nomura,a   Kyong-Hoon Lee,a   Kazunori Ikebukuroa  and  Isao Karube*a  

* Corresponding authors

a Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, Japan
E-mail: karube@bio.rcast.u-tokyo.ac.jp
Fax: +81 3 5452 5227
Tel: +81 3 5452 5220

Abstract

An enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD) is presented. This method is based on a competitive reaction between OCDD and OCDD-HRP (horseradish peroxidase) conjugate against the antibody, whereby OCDD-HRP is detected colorimetrically at 450 nm. The detection limit of OCDD was 0.78 µg mL−1. Optimizing the reaction conditions of the assay, cross reactivities of some dioxins against the antibody are discussed.

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Article information

DOI
https://doi.org/10.1039/B104258K
Article type
Communication
Submitted
15 May 2001
Accepted
14 Jun 2001
First published
29 Jun 2001

Analyst, 2001,126, 1207-1209

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Dioxin detection based on immunoassay using a polyclonal antibody against octa-chlorinated dibenzo-p-dioxin (OCDD)

M. Shimomura, Y. Nomura, K. Lee, K. Ikebukuro and I. Karube, Analyst, 2001, 126, 1207 DOI: 10.1039/B104258K

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