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Issue 8, 2001
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Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA

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Abstract

For immunization, sennoside B was conjugated with bovine serum albumin. The hapten density in the antigen conjugate was determined to be 3 mol mol−1 protein by matrix-assisted laser desorption–ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside B was produced by fusing splenocytes from mouse immunized with the sennoside B conjugate and mouse myeloma cells. Weak cross-reactivities occurred with sennoside A which is a stereochemical isomer, and a monomer of sennoside B, rhein, but no cross-reactivity was observed with other related anthraquinones and phenolics. The range of the assay extended from 0.5 ng ml−1 to 15 ng ml−1 of sennoside B, and good correlation between ELISA and HPLC methods was obtained when crude extracts of rhubarb were analyzed.

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Publication details

The article was received on 06 Apr 2001, accepted on 14 Jun 2001 and first published on 25 Jul 2001


Article type: Paper
DOI: 10.1039/B103153H
Citation: Analyst, 2001,126, 1372-1376
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    Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA

    O. Morinaga, S. Nakajima, H. Tanaka and Y. Shoyama, Analyst, 2001, 126, 1372
    DOI: 10.1039/B103153H

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