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Issue 6, 2001
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Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection

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Abstract

A high-performance liquid chromatographic method with electrochemical detection was developed for the determination of twelve tea catechins including four major catechins: epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG) and epigallocatechin gallate (EGCG); four of their epimers at the C-2 position, C, GC, CG and GCG; and four methylated catechin derivatives, epigallocatechin-3-O-(3-O-methyl)gallate, gallocatechin-3-O-(3-O-methyl)gallate, epigallocatechin-3-O-(4-O-methyl)gallate and epicatechin-3-O-(3-O-methyl)gallate. These catechins were separated on an ODS C18 reversed-phase column by isocratic elution with 0.1 M NaH2PO4 buffer (pH 2.5)–acetonitrile (87∶13) containing 0.1 mM EDTA·2Na. The detection limits (S/N = 3) of these catechins were approximately 10–40 pmol ml−1 at an applied voltage of 600 mV. Extracting these catechins from tea leaf powder with H2O–acetonitrile (1∶1) at 30 °C for 40 min inhibited the epimerization at C-2 significantly from these epicatechins compared to extraction with hot water at 90 °C. This analytical method is sensitive to and appropriate for the simultaneous determination of various biologically active catechins in green tea.

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Publication details

The article was received on 19 Mar 2001, accepted on 18 Apr 2001 and first published on 23 May 2001


Article type: Paper
DOI: 10.1039/B102541B
Citation: Analyst, 2001,126, 816-820
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    Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection

    M. Sano, M. Tabata, M. Suzuki, M. Degawa, T. Miyase and M. Maeda-Yamamoto, Analyst, 2001, 126, 816
    DOI: 10.1039/B102541B

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