Issue 8, 2000

On determining intramolecular distances from donor–donor energy migration (DDEM) within bifluorophoric macromolecules

Abstract

Recently a model based on donor–donor energy migration (DDEM) was developed for examining structure–function properties of biomacromolecules such as proteins (J. Chem. Soc., Faraday Trans., 1996, 92, 1563). Unlike the extended Förster theory (EFT; J. Chem. Phys., 1996, 105, 10896) the DDEM model is straightforward to apply in the analyses of fluorescence depolarisation experiments, as obtained by the time-correlated single photon counting (TCSPC) technique. In order to test the validity of the DDEM model, the EFT was used to create synthetic depolarisation data. These mimic true TCSPC experiments and cover a wide range of physical conditions, which are difficult to arrange for in real experiments with model systems. In particular, the relative rate of DDEM (ω) and the rotation correlation time (ϕ) of the donor molecules was examined. The DDEM rates obtained from the analyses were compared to the true rates. From these results the relative error of the intramolecular distances were calculated. For values 1<12ωϕ<25, the DDEM model is slightly overestimating the distances. Typically, the distances determined with the DDEM model are overestimated by 5–10%.

Article information

Article type
Paper
Submitted
09 Dec 1999
Accepted
18 Feb 2000
First published
29 Mar 2000

Phys. Chem. Chem. Phys., 2000,2, 1789-1794

On determining intramolecular distances from donor–donor energy migration (DDEM) within bifluorophoric macromolecules

P. Edman, P. Westlund and L. B.-Å. Johansson, Phys. Chem. Chem. Phys., 2000, 2, 1789 DOI: 10.1039/A909701E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements