Jump to main content
Jump to site search

Issue 8, 2000
Previous Article Next Article

On determining intramolecular distances from donor–donor energy migration (DDEM) within bifluorophoric macromolecules

Author affiliations

Abstract

Recently a model based on donor–donor energy migration (DDEM) was developed for examining structure–function properties of biomacromolecules such as proteins (J. Chem. Soc., Faraday Trans., 1996, 92, 1563). Unlike the extended Förster theory (EFT; J. Chem. Phys., 1996, 105, 10896) the DDEM model is straightforward to apply in the analyses of fluorescence depolarisation experiments, as obtained by the time-correlated single photon counting (TCSPC) technique. In order to test the validity of the DDEM model, the EFT was used to create synthetic depolarisation data. These mimic true TCSPC experiments and cover a wide range of physical conditions, which are difficult to arrange for in real experiments with model systems. In particular, the relative rate of DDEM (ω) and the rotation correlation time (ϕ) of the donor molecules was examined. The DDEM rates obtained from the analyses were compared to the true rates. From these results the relative error of the intramolecular distances were calculated. For values 1<12ωϕ<25, the DDEM model is slightly overestimating the distances. Typically, the distances determined with the DDEM model are overestimated by 5–10%.

Back to tab navigation

Publication details

The article was received on 09 Dec 1999, accepted on 18 Feb 2000 and first published on 29 Mar 2000


Article type: Paper
DOI: 10.1039/A909701E
Citation: Phys. Chem. Chem. Phys., 2000,2, 1789-1794
  •   Request permissions

    On determining intramolecular distances from donor–donor energy migration (DDEM) within bifluorophoric macromolecules

    P. Edman, P. Westlund and L. B.-Å. Johansson, Phys. Chem. Chem. Phys., 2000, 2, 1789
    DOI: 10.1039/A909701E

Search articles by author

Spotlight

Advertisements