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Issue 8, 2000
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Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye–DNA complexes

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Abstract

We report the fluorescence lifetimes of the DNA-stain dye PicoGreen and discuss the difference exhibited in the upon binding to single-stranded vs. double-stranded DNA; we here developed a direct method for determining single-to-double stranded DNA ratios in solution by measurement of the pre-exponential factors in the fluorescence decay traces of dye–DNA complexes.

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Publication details

The article was received on 28 Dec 1999, accepted on 03 Mar 2000 and first published on 30 Mar 2000


Article type: Communication
DOI: 10.1039/B000473L
Citation: Chem. Commun., 2000,0, 689-690
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    Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye–DNA complexes

    G. Cosa, K.-S. Focsaneanu, J. R. N. McLean and J. C. Scaiano, Chem. Commun., 2000, 0, 689
    DOI: 10.1039/B000473L

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