Jump to main content
Jump to site search

Issue 8, 2000
Previous Article Next Article

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Author affiliations

Abstract

A combination of ELISA, Western blotting and immunoaffinity concentration using an anti-ginsenoside Rb1 monoclonal antibody was applied for qualitative and quantitative surveys of ginsenoside Rb1 and related ginsenosides in roots and traditional Chinese herbal medicines. To improve the low correlation between ELISA and HPLC analysis, the crude extract of roots was immunoaffinity concentrated to make evident the effect of malonyl ginsenoside Rb1. Immunoaffinity column chromatography also concentrated an unknown ginsenoside which had the same cross-reaction with ginsenoside Rb1.

Back to tab navigation

Publication details

The article was received on 12 Apr 2000, accepted on 22 Jun 2000 and first published on 25 Jul 2000


Article type: Paper
DOI: 10.1039/B002932G
Citation: Analyst, 2000,125, 1425-1429
  •   Request permissions

    Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

    N. Fukuda, H. Tanaka and Y. Shoyama, Analyst, 2000, 125, 1425
    DOI: 10.1039/B002932G

Search articles by author

Spotlight

Advertisements