Jump to main content
Jump to site search

Issue 7, 2000
Previous Article Next Article

Measurement of the continuous distribution of binding sites in molecularly imprinted polymers

Author affiliations

Abstract

Reported is the first affinity spectrum (AS) [number of binding sites (N) vs. association constant (K)] for a non-covalently imprinted polymer. The AS method yields the distribution of sites over a continuous range of binding constants and characterizes the heterogeneity present in imprinted polymers better than current methodologies. To demonstrate the generality of the AS method, the distributions for three different imprinted polymers (two of which were taken from the literature) were calculated from their respective binding isotherms. The shapes of the distribution curves were different yet consistent with the respective covalent or non-covalent imprinting mechanisms. Finally, the binding parameters derived from the AS method were compared with those determined by the more common Scatchard analysis and were in general agreement.

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Mar 2000, accepted on 28 Apr 2000 and first published on 12 Jun 2000


Article type: Paper
DOI: 10.1039/B002354J
Citation: Analyst, 2000,125, 1261-1265
  •   Request permissions

    Measurement of the continuous distribution of binding sites in molecularly imprinted polymers

    R. J. Umpleby II, M. Bode and K. D. Shimizu, Analyst, 2000, 125, 1261
    DOI: 10.1039/B002354J

Search articles by author

Spotlight

Advertisements