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Issue 6, 2000
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Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter

Yordan Kostov,a  Peter Harms,a  Robert S. Pilatob  and  Govind Rao*a 
Author affiliations

* Corresponding authors

a Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725W. Lombard St., Baltimore, USA.
E-mail: grao@umbc.edu

b LUMET, 1808 Briggs Chaney Road, Silver Spring, USA

Abstract

A new method and device for the ratiometric measurement of oxygen concentration are presented. They are based on the use of a dual-emission oxygen-sensitive dye. The method allows the exclusion of the influence of emission overlap. The detection of the dual-emission ratio is performed using a single long-pass emission filter. The device described is simpler than the widely used lifetime instruments and could easily be a stand-alone low-cost instrument.

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Publication details

The article was received on 17 Feb 2000, accepted on 20 Mar 2000 and first published on 09 May 2000


Article type: Paper
DOI: 10.1039/B002348P
Citation: Analyst, 2000,125, 1175-1178
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    Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter

    Y. Kostov, P. Harms, R. S. Pilato and G. Rao, Analyst, 2000, 125, 1175
    DOI: 10.1039/B002348P

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