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Issue 3, 2000
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HPLC determination of catechins and caffeine in tea.Differentiation of green, black and instant teas

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Abstract

A simple and fast high performance liquid chromatographic method for five catechins and caffeine using an ODS column and a water–acetonitrile–formic acid mobile phase system was developed. The catechins (epicatechin, catechin, epigallocatechin, epigallocatechin gallate, epicatechin gallate) and caffeine were separated by an acetonitrile gradient within 20 min. The detection limit of the method was approximately 10 ng for all the compounds (by injecting 10 μL). Several green, black and instant teas were analysed using this method. By using the studied compounds as chemical descriptors, linear discriminant analysis was performed and complete differentiation of the green, black and instant teas was achieved.

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Publication details

The article was received on 22 Nov 1999, accepted on 05 Jan 2000 and first published on 07 Feb 2000


Article type: Paper
DOI: 10.1039/A909219F
Citation: Analyst, 2000,125, 421-425
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    HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas

    P. L. Fernández, M. J. Martín, A. G. González and F. Pablos, Analyst, 2000, 125, 421
    DOI: 10.1039/A909219F

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