Violet (405 nm) diode laser for laser induced fluorescence detection in capillary electrophoresis

Abstract

The first application of the violet diode laser for laser induced fluorescence (LIF) detection in capillary electrophoresis is reported. Operating at 405 nm with an output power of 5 mW, this laser offers many advantages over conventional red diode lasers operating at 635 nm. We demonstrate the suitability of the violet diode laser for LIF detection by detecting amino acids labeled with naphthalene-2,3-dicarboxaldehyde (NDA). Detection limits of 10 nM were achieved with a linear range of over three orders of magnitude.