Determination of thiamine and riboflavin in pet foods and animal feedingstuffs

Abstract

A method is given for the determination of thiamine (vitamin B₁) and riboflavin (vitamin B₂) in pet foods and animal feedingstuffs. The vitamins are extracted from the food matrix by digestion with 0.1 M hydrochloric acid followed by enzyme digestion with clara-diastase. The filtered solution is analysed by reversed-phase HPLC for riboflavin (directly) and thiamine (after thiochrome formation) using fluorescence detection.