Kalman filtering-aided time-resolved solid-surface room temperature phosphorimetry for simultaneous determination of anthracyclines in solution

Abstract

Daunorubicin, doxorubicin and epirubicin react with Eu^III to form complexes which exhibit analytically useful room temperature phosphorescence (RTP). The RTP features of the three complexes are similar and the RTP spectra completely overlap. However, their three phosphorescence decay rates are different, and these differences were utilized to analyse the time-resolved RTP data by Kalman filtering. Simultaneous quantification of all three complexes is demonstrated and a method is proposed for the simultaneous determination of the three anthracyclines in mixtures by RTP optosensing. The analytical errors observed are within ± 5%.