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Issue 10, 1997
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Systematic Deviations due to Random Noise Levels in Size Exclusion Chromatography Coupled With Multi Angle Laser Light Scattering

P. Tackx  and  F. Bosscher 

Abstract

Starting from a theoretical molar mass distribution (generalized exponential distribution), the concentration and light scattering chromatograms of a size exlusion chromatography coupled with multi angle laser light scattering experiment are constructed with random noise levels. From these noisy curves, a log M versus retention volume plot is created. This plot shows a systematic deviation in the low and high molar mass region. In the low molar mass region (poor light scattering signals), the log M signals are systematically too high. In the high molar mass region (poor concentration signals), the log M signals are constantly too low. We show that this systematic error is due to the fact that the logarithm of negative numbers is not defined.

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Article type: Paper
DOI: 10.1039/A705998A
Citation: Anal. Commun., 1997,34, 295-297
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    Systematic Deviations due to Random Noise Levels in Size Exclusion Chromatography Coupled With Multi Angle Laser Light Scattering

    P. Tackx and F. Bosscher, Anal. Commun., 1997, 34, 295
    DOI: 10.1039/A705998A

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