Indirect enzymatic phosphorylation: preparation of dihydroxyacetone phosphate

Paola D'Arrigo; Valentino Piergianni; Giuseppe Pedrocchi-Fantoni; Stefano Servi

doi:10.1039/C39950002505

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Journal of the Chemical Society, Chemical Communications

Issue 24, 1995

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Indirect enzymatic phosphorylation: preparation of dihydroxyacetone phosphate

Paola D'Arrigo , Valentino Piergianni , Giuseppe Pedrocchi-Fantoni and Stefano Servi

J. Chem. Soc., Chem. Commun., 1995, 2505-2506

DOI: 10.1039/C39950002505

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Abstract
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Starting from natural phosphatidyl choline, the substitution of the choline polar head for an alcohol donor is catalysed by phospholipase D; subsequent hydrolysis of the new phospholipid obtained in the presence of phospholipase C allows the obtainment of the phosphate of the alcohol donor; the overall process is the net transfer of the phosphate moiety from a phospholipid to a primary alcohol and the procedure is verified in the preparation of dihydroxyacetone phosphate

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Journal of the Chemical Society, Chemical Communications

Indirect enzymatic phosphorylation: preparation of dihydroxyacetone phosphate

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