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Labelling experiments with 2H and 3H prove that as cobyrinic acid is biosynthesised by the anaerobic bacterium Propionibacterium shermanii, its H–19 is derived from HR(and not HS) at C-4 of a reduced nicotinamide cofactor, so demonstrating that the biosynthetic pathway involves a reduction step.
K. Ichinose, M. Kodera, F. J. Leeper and A. R. Battersby, J. Chem. Soc., Chem. Commun., 1993, 515 DOI: 10.1039/C39930000515
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