A sensitive spectofluorimetric method has been developed for the determination of human serum albumin (HSA). The complex of Eriochrome Cyanine R (ECR) with HSA fluoresces at 423 nm (with excitation at 308 nm). The limit of determination is 0.5 µg ml–1 and the relative standard deviation is less than 2%. The composition of the fluorescent complex is 1 : 4 (HSA : ECR).
Y. Ci and L. Chen, Analyst, 1988, 113, 679 DOI: 10.1039/AN9881300679
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