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Issue 4, 1986
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Enzyme entrapment in electrically conducting polymers. Immobilisation of glucose oxidase in polypyrrole and its application in amperometric glucose sensors

Abstract

A technique is described for the entrapment of glucose oxidase in a polypyrrole matrix electrochemically deposited on a printed platinum electrode. The enzyme activity incorporated into the polymer was quantified by spectrophotometric assay using a flow-through cell and found to be proportional to the activity in the electropolymerisation medium. Polymer-entrapped glucose oxidase electrodes can be operated as amperometric glucose sensors in the presence or absence of soluble mediators. These electrodes respond rapidly, reaching a steady state within 20–40 s, with the enzymatic response being current limiting. The potential of this new immobilisation technique to the development of biosensors is discussed.

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Article type: Paper
DOI: 10.1039/F19868201259
Citation: J. Chem. Soc., Faraday Trans. 1, 1986,82, 1259-1264
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    Enzyme entrapment in electrically conducting polymers. Immobilisation of glucose oxidase in polypyrrole and its application in amperometric glucose sensors

    N. C. Foulds and C. R. Lowe, J. Chem. Soc., Faraday Trans. 1, 1986, 82, 1259
    DOI: 10.1039/F19868201259

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