The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quaternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a cross-linking entity

Paul M. Hardy; Graham J. Hughes; H. N. Rydon

doi:10.1039/P19790002282

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The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quaternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a cross-linking entity

Paul M. Hardy, Graham J. Hughes and H. N. Rydon

Abstract

1-(5-Amino-5-carboxypentyl)pyridinium chloride (3) and 1-(5-amino-5-carboxypentyl)-3-[1-(5-amino-5-carboxypentyl)-2-piperidyl]pyridinium chloride, anabilysine (4), have been isolated from acid hydrolysates of glutaraldehyde-treated ovalbumin and their structures confirmed by synthesis. It is concluded that the strong chromophore at ca. 265 nm shown by glutaraldehyde-treated proteins is due to the formation of such quaternary pyridinium compounds and that anabilysine residues are important in the cross-linking of proteins by glutaraldehyde.

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Article type: Paper

DOI: 10.1039/P19790002282

Citation: J. Chem. Soc., Perkin Trans. 1, 1979,0, 2282-2288

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The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quaternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a cross-linking entity

P. M. Hardy, G. J. Hughes and H. N. Rydon, J. Chem. Soc., Perkin Trans. 1, 1979, 0, 2282
DOI: 10.1039/P19790002282
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Journal of the Chemical Society, Perkin Transactions 1

The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quaternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a cross-linking entity

Abstract

The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quaternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a cross-linking entity

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