An improved colour reagent for the determination of blood glucose by the oxidase system

Denise Barham; P. Trinder

doi:10.1039/AN9729700142

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Analyst

Issue 1151, 1972

The home of premier fundamental discoveries, inventions and applications in the analytical and bioanalytical sciences

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An improved colour reagent for the determination of blood glucose by the oxidase system

Denise Barham and P. Trinder

Analyst, 1972,97, 142-145

DOI: 10.1039/AN9729700142

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A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.

The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.

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An improved colour reagent for the determination of blood glucose by the oxidase system

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