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Issue 11, 2014
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A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

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Abstract

An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus through ligation the terminal label can be transformed to an internal one.

Graphical abstract: A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

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Publication details

The article was received on 11 Aug 2013, accepted on 16 Oct 2013 and first published on 17 Oct 2013


Article type: Communication
DOI: 10.1039/C3CC46132G
Citation: Chem. Commun., 2014,50, 1313-1316
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    A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

    A. Samanta, A. Krause and A. Jäschke, Chem. Commun., 2014, 50, 1313
    DOI: 10.1039/C3CC46132G

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