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Issue 8, 2006
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Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

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Abstract

The ability to separate living cells is an essential aspect of cell research. Magnetic cell separation methods are among some of the most efficient methods for bulk cell separation. With the development of microfluidic platforms within the biotechnology sector, the design of miniaturised magnetic cell sorters is desirable. Here, we report the continuous sorting of cells loaded with magnetic nanoparticles in a microfluidic magnetic separation device. Cells were passed through a microfluidic chamber and were deflected from the direction of flow by means of a magnetic field. Two types of cells were studied, mouse macrophages and human ovarian cancer cells (HeLa cells). The deflection was dependent on the magnetic moment and size of the cells as well as on the applied flow rate. The experimentally observed deflection matched well with calculations. Furthermore, the separation of magnetic and non-magnetic cells was demonstrated using the same microfluidic device.

Graphical abstract: Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

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Publication details

The article was received on 29 Mar 2006, accepted on 13 Jun 2006 and first published on 03 Jul 2006


Article type: Paper
DOI: 10.1039/B604542A
Citation: Lab Chip, 2006,6, 974-980
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    Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

    N. Pamme and C. Wilhelm, Lab Chip, 2006, 6, 974
    DOI: 10.1039/B604542A

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