Jump to main content
Jump to site search

Issue 6, 2016
Previous Article Next Article

An electropolymerized molecularly imprinted polymer for selective carnosine sensing with impedimetric capacity

Author affiliations

Abstract

A chemosensor with a molecularly imprinted polymer (MIP) film as the recognition unit selective to a carnosine biomarker was molecularly engineered, devised and fabricated. The molecular structure of the pre-polymerization complex of the carnosine template with the carboxy and 18-crown-6 ether derivatives of bis(2,2′-bithien-5-yl)methane functional monomers was thermodynamically optimized by density functional theory (DFT) at the B3LYP/6-31g(d) level. The calculated high negative Gibbs free energy change, ΔG = −227.4 kJ mol−1, indicated the formation of a very stable complex. The solution of this complex was prepared and used for deposition of the MIP films on a Pt disk electrode or an Au electrode of the quartz crystal resonator by potentiodynamic electropolymerization. Subsequently, the carnosine template was extracted from the MIPs with 0.1 M NaOH, as confirmed by the differential pulse voltammetry (DPV), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy measurements. For carnosine sensing, impedimetric capacity (IC) measurements were performed under flow-injection analysis (FIA) conditions resulting in the limit of detection of 20 μM (at S/N = 3). This limit implied the readiness of the chemosensor for carnosine determination in clinical samples. Due to multiple modes of carnosine binding to MIP recognition sites, the IC chemosensor was found to be more selective to carnosine than to its common interferences including anserine, carcinine and histidine. Advantageously, the imprinting factor, determined by piezoelectric microgravimetry (PM), was high equaling 14.9.

Graphical abstract: An electropolymerized molecularly imprinted polymer for selective carnosine sensing with impedimetric capacity

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Oct 2015, accepted on 02 Jan 2016 and first published on 04 Jan 2016


Article type: Paper
DOI: 10.1039/C5TB02260F
Citation: J. Mater. Chem. B, 2016,4, 1156-1165
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    An electropolymerized molecularly imprinted polymer for selective carnosine sensing with impedimetric capacity

    A. Wojnarowicz, P. S. Sharma, M. Sosnowska, W. Lisowski, T. Huynh, M. Pszona, P. Borowicz, F. D'Souza and W. Kutner, J. Mater. Chem. B, 2016, 4, 1156
    DOI: 10.1039/C5TB02260F

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements