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Issue 36, 2016
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Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

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Abstract

A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.

Graphical abstract: Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

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Publication details

The article was received on 26 Feb 2016, accepted on 05 Apr 2016 and first published on 07 Apr 2016


Article type: Communication
DOI: 10.1039/C6CC01749E
Citation: Chem. Commun., 2016,52, 6158-6161
  • Open access: Creative Commons BY license
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    Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s

    M. J. Weissenborn, S. Notonier, S. Lang, K. B. Otte, S. Herter, N. J. Turner, S. L. Flitsch and B. Hauer, Chem. Commun., 2016, 52, 6158
    DOI: 10.1039/C6CC01749E

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