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Issue 8, 2011
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Influencing uptake and localization of aminoglycoside-functionalized peptoids

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Abstract

The development of small-molecule therapeutics that target RNA remains a promising field but one hampered with considerable challenges that include programming high affinity, specificity, cell permeability, and favorable pharmacokinetic profiles. Previously, we employed the use of peptoids to modularly display RNA-binding modules to enhance binding affinity and specificity by altering valency and the distance between ligand modules. Herein, factors that affect uptake, localization, and toxicity of peptoids that display a kanamycin derivative into a variety of mammalian cells lines are reported. A series of peptoids that display various spacing modules was synthesized to determine if the spacing module affects permeability and localization. The spacing module does affect cellular permeability into C2C12, A549, HeLa, and MCF7 cell lines but not into Jurkat cells. Moreover, the modularly assembled peptoids carrying the kanamycin cargo localize in the cytoplasm and perinuclear region of C2C12 and A549 cells and throughout HeLa cells, including the nucleus. These studies could contribute to the development of general strategies to afford cell permeable, modularly assembled small molecules that specifically target RNAs present in a variety of cell types.

Graphical abstract: Influencing uptake and localization of aminoglycoside-functionalized peptoids

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Publication details

The article was received on 24 Feb 2011, accepted on 04 May 2011 and first published on 24 May 2011


Article type: Paper
DOI: 10.1039/C1MB05074E
Citation: Mol. BioSyst., 2011,7, 2441-2451
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    Influencing uptake and localization of aminoglycoside-functionalized peptoids

    M. M. Lee, J. M. French and M. D. Disney, Mol. BioSyst., 2011, 7, 2441
    DOI: 10.1039/C1MB05074E

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