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Issue 17, 2016
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Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

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Abstract

A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.

Graphical abstract: Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

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Publication details

The article was received on 10 Mar 2016, accepted on 01 Apr 2016 and first published on 04 Apr 2016


Article type: Communication
DOI: 10.1039/C6OB00533K
Citation: Org. Biomol. Chem., 2016,14, 4023-4026
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    Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

    M. Liras, S. Simoncelli, A. Rivas-Aravena, O. García, J. C. Scaiano, E. I. Alarcon and A. Aspée, Org. Biomol. Chem., 2016, 14, 4023
    DOI: 10.1039/C6OB00533K

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