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Issue 6, 2016
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Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

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Abstract

We report a novel atomic force microscopy (AFM) technique with dual actuation capabilities using both piezo and magnetic bead actuation for advanced single-molecule force spectroscopy experiments. The experiments are performed by manipulating magnetic microbeads using an electromagnet against a stationary cantilever. Magnetic actuation has been demonstrated before to actuate cantilevers, but here we keep the cantilever stationary and accomplish actuation via free-manipulated microstructures. The developed method benefits from significant reduction of drift, since the experiments are performed without a substrate contact and the measured force is inherently differential. In addition, shrinking the size of the actuator can minimize hydrodynamic forces affecting the cantilever. The new method reported herein allows for the application of constant force to perform force-clamp experiments without any active feedback, profiled for a deeper understanding of biomolecular interactions.

Graphical abstract: Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

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Publication details

The article was received on 22 Jul 2016, accepted on 07 Sep 2016 and first published on 09 Sep 2016


Article type: Communication
DOI: 10.1039/C6NH00134C
Citation: Nanoscale Horiz., 2016,1, 488-495
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    Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements

    S. Sevim, S. Ozer, L. Feng, J. Wurzel, A. Fakhraee, N. Shamsudhin, B. Jang, C. Alcantara, O. Ergeneman, E. Pellicer, J. Sort, T. Lühmann, S. Pané, B. J. Nelson and H. Torun, Nanoscale Horiz., 2016, 1, 488
    DOI: 10.1039/C6NH00134C

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