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Issue 20, 2015
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Low-voltage paper isotachophoresis device for DNA focusing

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Abstract

We present a new paper-based isotachophoresis (ITP) device design for focusing DNA samples having lengths ranging from 23 to at least 1517 bp. DNA is concentrated by more than two orders of magnitude within 4 min. The key component of this device is a 2 mm-long, 2 mm-wide circular paper channel formed by concertina folding a paper strip and aligning the circular paper zones on each layer. Due to the short channel length, a high electric field of ~16 kV m−1 is easily generated in the paper channel using two 9 V batteries. The multilayer architecture also enables convenient reclamation and analysis of the sample after ITP focusing by simply opening the origami paper and cutting out the desired layers. We profiled the electric field in the origami paper channel during ITP experiments using a nonfocusing fluorescent tracer. The result showed that focusing relied on formation and subsequent movement of a sharp electric field boundary between the leading and trailing electrolyte.

Graphical abstract: Low-voltage paper isotachophoresis device for DNA focusing

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Publication details

The article was received on 25 Jul 2015, accepted on 21 Aug 2015 and first published on 04 Sep 2015


Article type: Paper
DOI: 10.1039/C5LC00875A
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2015,15, 4090-4098
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    Low-voltage paper isotachophoresis device for DNA focusing

    X. Li, L. Luo and R. M. Crooks, Lab Chip, 2015, 15, 4090
    DOI: 10.1039/C5LC00875A

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