Jump to main content
Jump to site search

Issue 81, 2014
Previous Article Next Article

Monitoring ATP hydrolysis and ATPase inhibitor screening using 1H NMR

Author affiliations

Abstract

We present a versatile method to characterize ATPase and kinase activities and discover new inhibitors of these proteins. The proton NMR-based assay directly monitors ATP turnover and is easy to implement, requires no additional reagents and can potentially be applied to GTP. We validated the method's accuracy, applied it to the monitoring of ATP turnover by actin and to the screening of ATPase inhibitors, and showed that it is also applicable for the monitoring of GTP hydrolysis.

Graphical abstract: Monitoring ATP hydrolysis and ATPase inhibitor screening using 1H NMR

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Jun 2014, accepted on 22 Aug 2014 and first published on 29 Aug 2014


Article type: Communication
DOI: 10.1039/C4CC04399E
Citation: Chem. Commun., 2014,50, 12037-12039
  •   Request permissions

    Monitoring ATP hydrolysis and ATPase inhibitor screening using 1H NMR

    B. Guo, P. S. Gurel, R. Shu, H. N. Higgs, M. Pellegrini and D. F. Mierke, Chem. Commun., 2014, 50, 12037
    DOI: 10.1039/C4CC04399E

Search articles by author

Spotlight

Advertisements