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Issue 10, 2013
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Naturally occurring Ngn2 promoter activators from Butea superba

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Abstract

Neurogenin2 (Ngn2), an activator-type bHLH transcriptional factor, promotes differentiation of neural stem cells into neurons by transcription of pro-neural genes. To find neural stem cell accelerators from the extract library of natural resources, we used a two-step screening including a Ngn2 promoter reporter gene screening and differentiation assay screening of neural stem cells. A reporter gene assay that can detect Ngn2 promoter activity by luciferase expression was constructed using C3H10T1/2 cells. Using this primary cell-based screening, Butea superba was found to include Ngn2 promoter activators from our tropical plant extract libraries. Bioassay-guided fractionation of this plant extract led to the isolation of 18 natural products, including pterocarpans and isoflavonoids. Dehydromaackiain (1), formononetin (6), (−)-variabilin (13), (−)-medicarpin (14), rothindin (17) and ononin (18) showed 1.8–2.8 times higher Ngn2 promoter activity at 5 μM compared with control. Of active natural compounds, 3′-methoxydaidzein (3) showed promotion of neurite outgrowth of C17.2 in a secondary screen. 3′-Methoxydaidzein (3) increased mRNA expression of pro-neural transcriptional factors (Ngn2, Ngn1, NeuroD2), a mature neuron-specific enzyme GAD1 and a pro-neural neurotrophic growth factor neurotrophin 3 (NT3) in C17.2 neural stem cells.

Graphical abstract: Naturally occurring Ngn2 promoter activators from Butea superba

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Publication details

The article was received on 28 Feb 2013, accepted on 02 Jul 2013 and first published on 05 Jul 2013


Article type: Paper
DOI: 10.1039/C3MB70083F
Citation: Mol. BioSyst., 2013,9, 2489-2497
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    Naturally occurring Ngn2 promoter activators from Butea superba

    M. A. Arai, K. Koryudzu, T. Koyano, T. Kowithayakorn and M. Ishibashi, Mol. BioSyst., 2013, 9, 2489
    DOI: 10.1039/C3MB70083F

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